Fig. 1.

Ouabain-induced cyclooxygenase (COX)-2 expression and protein kinase A (PKA) activation in human lung fibroblasts (HLF). Serum-starved HLFs were treated with 100 nM ouabain with or without 1 μM NS-398 for the times indicated. Cells were analyzed by real-time qPCR for COX-2 mRNA levels (A) or by Western blotting for COX-2 expression and vasodilator-stimulated phosphoprotein (VASP) shift (A–C). C: HLFs were transfected with a scramble or COX-2 small-interfering RNAs (siRNAs), serum-starved for 24 h, and treated with 100 nM ouabain for 24 h. Shown are the Western blots for COX-2 and VASP.