Fig. 2.

Effect of ouabain, nicardipine, and KB-R7943 on ⁴⁵Ca²⁺ influx and on COX-2 expression and VASP shift. A: HLFs were preincubated for 24 h in the presence of 100 nM ouabain, 1 mM nicardipine, or 3 mM KB-R7943, and the rate of ⁴⁵Ca²⁺ influx was measured as described in experimental procedures. Means ± SE from experiments performed in triplicate are shown. B: HLFs were treated with 100 nM ouabain, 1 mM nicardipine, or 3 mM KB-R7943 for 24 h, and cell extracts were examined by Western blotting for COX-2 expression and VASP shift.