Fig. 8.

CaSR expression is upregulated and extracellular Ca²⁺-induced Akt phosphorylation and cell proliferation are enhanced in PASMC from IPAH patients. A: summarized data (means ± SE) showing CaSR protein expression level (determined by Western blot analysis) in normal and IPAH-PASMC. B: Western blot analysis of phosphorylated Akt (p-Akt) in normal and IPAH-PASMC treated with 0 or 0.5 mM extracellular Ca²⁺ for 5 or 13 min. C: summarized data (means ± SE) showing the no. of cells in the DNA synthesis (S) phase in normal and IPAH-PASMC in the absence (control) or presence of the TRPC channel blocker 2-aminoethoxydiphenyl borate (2-APB; 100 μM) or the CaSR blocker NPS-2143 (1 or 2 μM). **P < 0.01 and ***P < 0.001 vs. control in normal and IPAH PASMC.