Fig. 6.

αSMA-directed deletion of αv integrins does not confer protective effects against unilateral ureteral obstruction (UUO) induced renal fibrosis. A: immunofluorescence micrographs and image quantification of kidney sections from 7-day UUO-treated Ai14;αSMA-rtTA;tetO-Cre mice, costained with PDGFRβ antibody. Panel at left shows DAPI (blue) and endogenous tdTomato (i.e., αSMA) (red), panel in middle shows DAPI (blue) and PDGFRβ staining (green), and panel at right shows the merged image. Scale bar, 50 μm. B: hydroxyproline assay of kidney lysates. C: picrosirius red staining of kidney sections from mice that underwent UUO or sham operation (control) on their left kidneys for 14 days. Data are means ± SE. P value, Student's t-test. Scale bar, 50 μm.