Table 1.

Primers for PCR analysis

Gene/Primers
Dog COL1A1
F: 5′-AGAGGAGGGCCAAGAAGAAG-3′
R: 5′-CTCGGGTTTCCATACGTCTC-3′
Dog COL1A2
F: 5′-GCCCAGTATGATGGAAAAGG-3′
R: 5′-CAGGTCCTTGGAAACCTTGA-3′
Dog COL3A1
F: 5′-TCCTGGTATTCCTGGGAGAA-3′
R: 5′-TAGGACTCGAACTGGGGAGA-3′
Dog TGFB1
F: 5′-GAGCAGCATGTGGAGCTGTA-3′
R: 5′-CACGACTCCAGTGACATCAAA-3′
Dog MMP2
F: 5′-CACGGCCAACTATGATGATG-3′
R: 5′-CAGAATGCTCCAGTCCCATT-3′
Dog POSTN
F: 5′-GCCATCTGTGGAAGGAAAAC-3′
R: 5′-TCCCACAATGCCCAGAGTA-3′
Dog FN1
F: 5′-GAGGGGAGTGGAAGTGTGAA-3′
R: 5′-AGTAAACCACGCCACTGTCC-3′
Dog CTGF
F: 5′-TGTGAAGCTGACCTGGAAGA-3′
R: 5′-CCACAGAACTTAGCCCGGTA-3′
Dog HPRT
F: 5′-CAGACTTTGCTTTCCTTGGTCA-3′
R: 5′-CAGGTTTATAGCCAACACTTCGA-3′

F, forward; R, reverse; COL1A1, collagen type I α1; COL1A2, collagen type I α2; COL3A1, collagen type III α1; TGFB1, transforming growth factor-β1; MMP2, matrix metalloproteinase-II; POSTN, periostin; FN1, fibronectin; CTGF, connective tissue growth factor; HPRT, hypoxanthine guanine phosphoribosyl transferase.