Multiple-sequence alignment of the Vip3A proteins. Sequence identity is indicated by shading, where violet is 100% sequence identity, pale blue is 80 to 100%, yellow is 60 to 80%, and white is <60%. SP, signal peptide (50); “T,” 65-kDa fragment after proteolysis; “PPS1” and “PPS2,” first and second processing sites, respectively (50). Intervals of 10 amino acids are marked with “*.” The protein sequences used in this analysis are as follows: Vip3Aa1 (GenBank accession number AAC37036), Vip3Ab1 (accession number AAR40284), Vip3Ac1 (named PS49C; Seq. ID no. 7 in Narva and Merlo, U.S. patent application 20,040,128,716), Vip3Ad2 (accession number CAI43276), Vip3Ae1 (accession number CAI43277), Vip3Af1 (accession number CAI43275), Vip3Ag2 (accession number ACL97352), Vip3Ah1 (accession number ABH10614), Vip3Ai1 (accession number KC156693), Vip3Aj1 (accession number KF826717), Vip3Ba1 (accession number AAV70653), Vip3Bb2 (accession number ABO30520), and Vip3Ca1 (accession number ADZ46178).