Table 1.
Antibody overview
| Protein Target | Ab cat. Number or Name | Company or Donor | Ab Source | Migration, MW |
|---|---|---|---|---|
| 4E-BP1 | 9452 | Cell Signaling Technology | rabbit | 15–20 kDa |
| 4E-BP1 Thr-37/46 phos | 2855 | Cell Signaling Technology | rabbit | 15–20 kDa |
| ACCβ Ser-221 phos | 07–303 | Millipore | rabbit | 259 kDa |
| Actin | A2066 | Sigma Aldrich | rabbit | 42 kDa |
| AMPKα2 | AMPK α2 | Dr. J. Birk, University of Copenhagen | sheep | 63 kDa |
| AMPKα Thr-172 phos | 2531 | Cell Signaling Technology | rabbit | 63 kDa |
| CaMKII | 611293 | BD Transduction Laboratories | mouse | 55–75 kDa |
| CaMKII Thr-286 phos | 3361 | Cell Signaling Technology | rabbit | 55–75 kDa |
| eEF2 | ab130187 | Abcam | mouse | 95 kDa |
| eEF2 Thr-56 phos | 2331 | Cell Signaling Technology | rabbit | 95 kDa |
| FXYD1 | 13721-1-AP | Proteintech | rabbit | 12 kDa |
| FXYD1 unphosphorylated | AB_FXYD1–C2 | Dr. J. Randall Moorman, University of Virginia | rabbit | 12 kDa |
| FXYD1 Ser-68 phos | AB_FXYD1 ser68–CP68 | Dr. D. Bers, Loyola University | rabbit | 12 kDa |
| FXYD1 Ser-63 phos | FXYD1 Ser-63 phos | Professor M. Shattock, King's College London | rabbit | 12 kDa |
| FXYD1 Ser-68 phos | FXYD1 Ser-68 phos | Professor M. Shattock, King's College London | rabbit | 12 kDa |
| FXYD1 Thr-69 phos | FXYD1Thr-69 phos | Professor M. Shattock, King's College London | sheep | 12 kDa |
| mTOR | 2972 | Cell Signaling Technology | rabbit | 289 kDa |
| mTOR Ser-2448 phos | 2971 | Cell Signaling Technology | rabbit | 289 kDa |
| p70S6K1 | 2708 | Cell Signaling Technology | rabbit | 70 kDa |
| p70S6K1 Thr-389 phos | 9234 | Cell Signaling Technology | rabbit | 70 kDa |
| PKCα/β Thr-638/641 phos | 9375 | Cell Signaling Technology | rabbit | 80–82 kDa |
| PLN | PA5-19351 | Pierce–ThermoScientific | goat | 6 kDa |
| PLN Thr-17 phos | Sc-17024 | Santa Cruz Biotechnology | rabbit | 6 kDa |
4E-BP1, eukaryotic initiation factor 4E-binding protein 1; ACCβ Ser-221 phos, Acetyl-CoA carboxylase β serine-221 phosphorylation; AMPKα2, AMP-activated protein kinase α2; CaMKII, Ca2+/calmodulin-dependent protein kinase II; eEF2, eukaryotic elongation factor 2; FXYD1, phospholemman; mTOR, mammalian target of rapamycin; PKCα/β Thr-638/641 phos, protein kinase Cα/β threonine 638/641 phosphorylation; p70S6K1, ribosomal protein S6 p70 kinase 1; PLN, phospholamban.