Fig. 3.

Nitrite production in COS7 and mIMCD-3 cells. A: COS7 cells were transfected with NOS1β ± DNM2 and DNM2 significantly reduced nitrite production (n = 3, *P = 0.007). B: mIMCD-3 cells overexpressing DNM2 produced significantly less nitrite compared with vector control cells (n = 3; *P = 0.04). C: acute stimulation of transfected COS7 cells resulted in a significant increase in nitrite in the NOS1β transfected cells, but no significant effect in the NOS1β+DNM2 cells. *P < 0.05 compared with vehicle. †P < 0.05 compared with corresponding NOS1β vehicle or ionomycin treated cells.