Table 1.

qPCR summary

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Product, bp	Annealing, °C	SK3−/−/WT
Cre	GCGGTCTGGCAGTAAAAACTATC	GTGAAACAGCATTGCTGTCACTT	100	56	18.45 ± 1.39
SK3	GCTCTGATTTTTGGGATGTTTG	CGATGATCAAACCAAGCAGG	148	55	0.10 ± 0.03
IK1	TGCACGCTGAGATGTTGTGG	GTGTCTGTGAGGTGCCCCGT	584	63	1.61 ± 0.34
TRPV4	CGTCCAAACCTGCGAATGAAGTTC	CCTCCATCTCTTGTTGTCACTGG	186	60	1.25 ± 0.02
Cav1	GGGCAACATCTAGAAGCCCAACAA	CTGATGCACTGAATTCCAATCAGGAA	371	53	1.39 ± 0.07
GADPH	GGTGCCAACCCCAAACGTAT	CTTTCACAGCCTCCTTGATAGCA	88	51

qPCR conditions and results are presented with list of primer sequences, size of PCR products, programmed annealing temperatures for the primers used in this study, as well as the normalized results comparing mRNA expression in SK3^−/− and wild-type (WT) mesenteric arteries using ΔΔC_T comparative method. n = 4 mice from each genotype.