Fig. 2.
MSEW induced NADPH oxidase subunit expression and activity in aorta of adult male mice. Quantitative RT-PCR analysis of Cybb (NOX2; A), Nox4 (NOX4; B), Ncf2 (p67phox; C), and Ncf1 (p47phox; D) in aorta of control (n = 3–5) and MSEW (n = 4–9) mice. *P < 0.05. E: apocynin-inhibitable superoxide production by aortae from control (n = 4) and MSEW (n = 4) mice. F: aortic relaxation in response to acetylcholine (ACh) in presence of vehicle (VEH) or apocynin (APO, 300 μM). Refer to Table 2 for statistical analyses (n = 5–7 per group). †P < 0.05, †††P < 0.001 vs. MSEW APO. G: aortic relaxation in response to sodium nitroprusside (SNP) in presence of vehicle (VEH) or apocynin (APO, 300 μM). Refer to Table 2 for statistical analyses (n = 5–7 per group).