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. 2015 Sep 30;24(6):864–870. doi: 10.1038/ejhg.2015.213

Figure 3.

Figure 3

Ex vivo splicing assay of SMN1, SMN2, and hybrid SMN1E7/SMN2E8 minigenes. The mutant minigenes were constructed by PCR-based site-directed mutagenesis and transfected into the HEK293 cells. The picture on the left shows the products of RT-PCR on ethidium bromide-stained agarose gel. The quantitative results are shown on the right. Error bars indicate the SD; P-values were calculated by Student's t-test (**P<0.01, *P<0.05). The c.863T minigenes showed increased exon 7 skipping; the c.863A minigenes showed a slight increase of exon 7 exclusion; the c.863C minigenes showed increased inclusion of exon 7. (a) SMN1 minigenes; (b) SMN2 minigenes; (c) hybrid SMN1E7/SMN2E8 minigenes.