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. 2015 Sep 23;24(6):823–829. doi: 10.1038/ejhg.2015.214

Figure 1.

Figure 1

(a) Western blot analysis of cell lysates isolated from transfected HEK293 cells with pCMV-RET-WT or pCMV-RET-mutants: p.(Arg79Trp), p.(Arg144His), p.(Pro270Leu), p.(Arg694Gln), p.(Ala756Val), p.(Gly533Ser), p.(Tyr1062Cys), p.(Asp489Asn). RET variants p.(Leu56Met) and p.(Tyr1062Phe) were included as controls. (b) Densitometry analysis of p-RET and RET. Values were normalized using GFP and β-actin. (c) Densitometry analysis of p-ERK and RET. Values were normalized using GFP and β-actin. The expression of p-RET or p-ERK is showed as a fold change to RET expression. Three independent experiments were performed (N=3). (*P<0.05, **P<0.01, (−): without GDNF treatment, (+): with GDNF treatment).