Table 1. Primer pairs and amplicon information (see also Table 2 and Figure 1).

Primer pair sequence	Amplicon information
1F: 5′-GATAGGTTGTATATGTTGGTG-3′	Minus-RT primers: 180 bp
1R: 5′-CTAAGATCAGTGCCACAA-3′
2F: 5′-CAAACCGGACCTCACAGCAG-3′ (ex4)	GFAP: α,β,γ: 789 bp; ζ: 1073 bp; Δ135: 654 bp; Δ164: 625 bp; Δex6: 568 bp; Δex7: 745 bp; X4: 909 bp
2R: 5′-CCGATGGAGCCTAGAGCAGCAA-3′ (3′-UTR ex9)
3F: 5′-GGCTGCGGCTAGACCAACTCACTGC-3′ (ex1)	GFAP: α,β: 919 bp
3R: 5′-TCCTTGTGCTCCTGCTTGGA-3′ (ex9)
4F: 5′-CCTTCACTCCTCCTGTCATTCCA-3′ (5′-UTR ex2a)	GFAP: γ: 1121 bp
4R: 5′-CCGATGGAGCCTAGAGCAGCAA-3′ (3′-UTR ex9)
5F: 5′-GCAGTGAGTGTATGTTGGTGAAAGTG-3′ (5′-UTR ex2a)	GFAP: γ: 344 bp
5R: 5′-GACGGGCTAAGGTGGCTTCA-3′ (ex3)
6F: 5′-GGCTGGAGGAGGAGGGACAGAA-3′ (ex6)	GFAP: δ/ɛ: 367 bp; κ: 739 bp
6R: 5′-GGGCCTGGTGAGGCTTGCT-3′ (3′-UTR ex7a/7b)
7F: 5′-GCATCGCCCACCAGCACCT-3′ (promoter)	GFAP: gDNA: 812 bp (ex 1)
7R: 5′-GGGTAAACAGAGGAGCCAAGGATCAC-3′ (in1)
8F: 5′-CAAACCGGACCTCACAGCAG-3′ (ex4)	GFAP: gDNA: 181 bp (GFAP variant c.719G>A detection assay)
8R: 5′-CCCACCCTTATTCCCTGATTCTC-3′ (in4)