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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: Nat Biotechnol. 2015 Jan 5;33(4):377–383. doi: 10.1038/nbt.3095

Figure 1.

Figure 1

A competitive E. coli – S. cerevisiae consortium for production of oxygenated taxanes. (a) Both E. coli TaxE1 and the yeast TaxS1 grew on glucose; E. coli TaxE1 produced taxadiene which can diffuse to the yeast, where it is oxygenated. (b) Only the co-culture produced the oxygenated taxanes. (c) Growth of E. coli TaxE1 was inhibited by the presence of the yeast. (d) The taxane productivity of E. coli TaxE1 was compromised by the presence of the yeast. Total taxanes = Taxadiene + Oxygenated taxanes. (e) These inhibitions could be due to the ethanol produced by the yeast, which was confirmed by follow-up experiments (Supplementary Fig. 2). Error bars, s.e. in all graphs (some error bars are smaller than the plot symbols). All replicates have also been plotted in all graphs (open circle), which indicates the number of replicates for each experiment.