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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: Nat Biotechnol. 2015 Jan 5;33(4):377–383. doi: 10.1038/nbt.3095

Figure 3.

Figure 3

Optimizing the yeast growth and engineering the yeast promoters improved production of the oxygenated taxanes. (a) Growth optimization (increasing the yeast inoculum and feeding additional nutrients) improved production of the oxygenated taxanes by more than two-fold. (b) A stronger promoter (UAS-GPDp), compared to the previously used TEFp, was found in the promoter screening in terms of taxadiene oxygenation. (c) The co-culture using UAS-GPDp also produced significantly (p<0.01, based on Student’s t-test) more oxygenated taxanes than that using TEFp. Error bars, s.e. in all graphs (some error bars are smaller than the plot symbols). All replicates have also been plotted in all graphs (open circle), which indicates the number of replicates for each experiment.