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. 2016 May 16;6:25944. doi: 10.1038/srep25944

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(a) HSMM were transfected with a siRNA against NOR-1 (siNOR-1; black bars) or a random siRNA (siRd; white bars). The expression of NOR-1 and that of SMPX in these samples was analyzed by real-time PCR (upper panel) (n = 6). *P < 0.0001 vs. siRd. The down-regulation of SMPX by siNOR-1 was also analyzed by Western blot (lower panel). Levels of β-actin were used as a loading control. (b) HSMM were differentiated to myotubes, and then were transfected with siNOR-1 or siRd. NOR-1 and SMPX mRNA levels (upper panel) and protein levels (lower panel) were analyzed as indicated in (a) (n = 6). *P < 0.0001, and ^#P = 0.0002 vs. siRd.