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. 2016 May 16;6:25944. doi: 10.1038/srep25944

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(a) Microphotographs from HSMM maintained in normal growth medium (Non-differentiated; ND) or exposed to differentiation medium (Differentiated; D) to induce myotube formation. (b) mRNA levels of NOR-1 and SMPX in non-differentiated HSMM (white bars) and differentiated cells (black bars) (n = 6). *P < 0.0001 vs. ND. (c,d) Protein levels of NOR-1 and SMPX analyzed by Western blot (c) and immunocytochemistry (d) in cells as indicated in (a). Levels of β-actin were used as a loading control in Western blot experiments. In (d), NOR-1 (green), SMPX (green) and nuclear staining (blue) are shown. Bars: 25 μm.