Skip to main content
. 2016 May 16;6:25944. doi: 10.1038/srep25944

Figure 6. NOR-1 knockdown prevents the binding of NOR-1 to SMPX promoter.

Figure 6

HSMM cells were transfected or not with a siRNA against NOR-1 (siNOR-1) or a control random siRNA (siRd), and then were exposed to differentiation conditions during five days (black bars). Non-differentiated HSMM were used as a control (white bars). The relative in vivo binding of NOR-1 to the human SMPX promoter was analyzed by ChIP assays. Immunoprecipitations were performed with an antibody against NOR-1 (IP:NOR-1) or a non-specific IgG (IP:IgG). The bars graph shows the levels enrichment of NOR-1 quantified by real-time PCR using SMPX promoter specific primers. Data were normalized to the total input DNA (n = 6). *P < 0.0001 vs. non-differentiated HSMM; #P < 0.0001 vs. differentiated HSMM transfected or not with siRd. The bottom panel shows the agarose gel electrophoresis of PCR products. Equal input DNA and control IgG immunoprecipitations are shown.