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. 2016 May 16;6:25944. doi: 10.1038/srep25944

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(a) Microphotographs from HSMM transfected with a siRNA against SMPX (siSMPX) or a control random siRNA (siRd) and then exposed to differentiation medium during five days. (b) SMPX mRNA levels (upper panel) and protein levels of SMPX, MyHC and p27 (lower panel) in HSMM treated as indicated in (a) (n = 6). *P < 0.0001 vs. siRd. Levels of β-actin were used as a loading control. (c) Immunocytochemistry for MyHC and phalloidin-stained F-actin in cells treated as indicated in (a). Bars: 25 μm.