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. 2016 May 16;6:25629. doi: 10.1038/srep25629

Figure 4. The levels of F-actin accumulation and phosphorylated WASP increase in the activation sites of CD23 KO B cells.

Figure 4

Splenic B cells from WT and CD23 KO mice were incubated with AF546-mB-Fab’-anti-Ig tethered to lipid bilayers at 37 °C for indicated times. Cells were fixed, permeabilized, and stained for F-actin and phosphorylated WASP (pWASP). Cells were analyzed using TIRFm. Shown are representative images of cells at 7 min (A,C) and the average MFI (±SD) in the B-cell contact zone (B,D) determined from 80 cells of three independent experiments. Scale bar, 2.5 μm. *p < 0.05.