Table 1. Experimental treatments: daily food added to C. finmarchicus adult females incubated in 1.5-L containers and harvested at one of two time points (2 or 5 days) for RNA-Seq.
Treatment |
|||
---|---|---|---|
ControlRhodomonas sp. | Low dose (LD) Rhodomonassp. Alexandrium fundyense | High dose (HD)Alexandrium fundyense | |
Target % by amountof carbon* | 100% Rho | 75% Rho, 25% Alex | 100% Alex |
Cells mL−1 | 8000 | Rho = 6000 | 200 |
Alex = 50 | |||
Total = 6050 | |||
μg C L−1* | 304 | Rho = 228 | 358 |
Alex = 89 | |||
Total = 317 | |||
Average daily toxiningestion (ng STXequivalents d−1)** | 0 | 0.3 | 2 |
*Cellular carbon content for Rhodomonas sp. and Alexandrium fundyense were computed applying volume to carbon conversions10.
**A. fundyense toxicity in stock cultures was measured daily during the duration of the experiment, and toxin ingestion was calculated from grazing rate experiments10, which were conducted in parallel to the incubations for the RNA-Seq experiment.