Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 16;6:25844. doi: 10.1038/srep25844

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) MS/MS spectra validating the phosphorylation of ACSS2 at serine 263 (b) WT ACSS2 and mutant ACSS2 S263A were expressed in 3T3-L1 adipocytes and treated with 1 μM Dex followed by insulin stimulation. Western blot analysis of insulin resistance measured using pAKT (S473) and pAKT (S308). Flag indicates expression levels of WT ACSS2 and ACSS2 S263A mutant. Actin served as loading control (n = 2 biological replicates). (c) Fold increase in triglyceride level in 3T3-L1 adipocytes overexpressing WT ACSS2 and mutant ACSS2 S263A upon Dex treatment (n = 2 biological replicates).