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. 2016 May 16;6:26013. doi: 10.1038/srep26013

Figure 2. Representative images of immunofluorescent labelling of neurons, axons and mitochondrial proteins in passively cleared 250μm mouse cerebellum.

Figure 2

Passively cleared 250 μm wild type mouse cerebellum sections were stained for both neuronal and mitochondrial proteins commonly found in the cerebellum. Calbindin ((a); red; 546 nm) and parvalbumin ((b); purple; 647 nm) antibodies positively label Purkinje cell bodies. Purkinje cell axons were visualised through positive labelling of neurofilaments ((c); red; 546 nm; NF-H, and (d); red; 546 nm; SMI-31) and myelin ((e); purple; 647 nm; MBP). Mitochondria were detected through positive labelling of COXI protein ((f); purple; 647 nm; COXI). Scale bar: 100 μm.