Figure 5. Representative images of Purkinje cells and their myelinated axons in human cerebellum following quadruple immunofluorescence.

Passively cleared 250 μm thick human cerebellar sections were stained for neurofilament H (blue), NDUFA13 (red), COX4 (green) and Myelin basic protein (purple). Mitochondria are clearly visible through the positive labelling of NDUFA13 (red) and COX4 (green). The myelin sheaths (Myelin basic protein, 647) of the Purkinje cell axons have been clearly labelled. In the 405 nm channel there is a high level of autofluorescence making it difficult to visualise Purkinje cell axons (neurofilament H). The presence of autofluorescence in the 405 nm and 488 nm channel was confirmed through a no primary antibody control section. Scale: 100 μm.