Figure 6. Reusing passively cleared and stained 250 μm thick control human cerebellum sections.

Passively cleared sections were originally stained for α-smooth muscle actin (purple; 647 nm), neurofilament H (green; 488 nm) and Glut-1 (red; 546 nm) producing a clear image of the cerebellar vascular network (a). Following a week long incubation in the clearing solution, the antibodies were removed and the sections were then successfully stained with COX1 (purple; 647 nm), neurofilament H (green; 488 nm) and myelin basic protein (red; 546 nm) demonstrating the distribution of mitochondria in myelinated axons (b). Scale: 100 μm.