Figure 4. Transient receptor potential canonical 3 (TRPC3) channel blocker inhibited fasiglifam-induced [Ca²⁺]_i increase.

(a) Fasiglifam (10 μM) increased intracellular Ca²⁺ concentration ([Ca²⁺]_i) and pyrazole-3 (Pyr3, 10 μM) inhibited fasiglifam-induced [Ca²⁺]_i increase at 8.3 mM glucose. At the end of the experiment, 100 μM tolbutamide (Tolb) was added to confirm that the responsive cells were beta-cell. (b) Pyr3 at 10 μM attenuated fasiglifam-induced [Ca²⁺]_i increases in single mouse beta-cell. (n = 16, number of single mice beta-cells examined in each group); ^∗P < 0.01 vs. fasiglifam.