Figure 4. SOD2 overexpression promotes the migration and invasion SACC.

To characterize the function of SOD2 in aiding metastasis, lentivirus containing SOD2 overexpression was transfected into SACC-83 cells. SOD2 protein concentrations and activities were significantly higher in the SOD2 overexpression-transfected SACC-83 cells than in the vector control transfected cells (A,B). SOD2 overexpression promoted the migration and invasion of SACC-83 cells (C,D). SACC-83 cells significant increased production of H₂O₂ after SOD2 overexpression (E). CAT protein concentrations and activity in SACC-83 cells did not differ after SOD2 overexpression (B,F). Vimentin, Slug, Snail, ERK, p-ERK, and MMP-2 protein concentrations were higher in SACC-83 cells, and E-cadherin protein concentrations were lower with SOD2 overexpression (B). *P < 0.05.