Table 2. Total and differential leukocyte counts, total T-cell subset (CD4+ and CD8+) counts, total CD4+ and CD8+ subset counts, the CD4:CD8 T-cell ratio, and the total number of T-cells responding to CMV (pp65; IE-1) and EBV (LMP-2; BMLF-1) antigens in peripheral blood before (PRE) and immediately after (POST) 30-minutes of steady state cycling exercise (n = 10).
| Cell Subset (cells/μL) | PRE | POST |
|---|---|---|
| Leukocytes | 5538 ± 1121 | 9888 ± 2205 # |
| Lymphocytes | 2006 ± 237 | 4075 ± 808 # |
| Monocytes | 425 ± 158 | 1025 ± 103 # |
| Granulocytes | 3163 ± 903 | 4875 ± 153 # |
| CD3+ T-cells | 1465 ± 171 | 2491 ± 569 # |
| CD4+ T-cells | 840 ± 181 | 1201 ± 274 # |
| CD4+ NA | 468 ± 157 | 613 ± 238 # |
| CD4+ CM | 265 ± 63 | 391 ± 110 # |
| CD4+ EM | 99 ± 66 | 162 ± 98 # |
| CD4+ EMRA | 18 ± 31 | 43 ± 67 |
| CD8+ T-cells | 450 ± 126 | 968 ± 465 # |
| CD8+ NA | 251 ± 66 | 401 ± 106 # |
| CD8+ CM | 36 ± 14 | 79 ± 38 |
| CD8+ EM | 73 ± 51 | 211 ± 172 # |
| CD8+ EMRA | 89 ± 61 | 277 ± 226 # |
| CD4:CD8 Ratio | 2.01 ± 0.72 | 1.52 ± 0.73 |
| CMV pp65 SFC | 4.8 ± 5.8 | 10.2 ± 10.8 |
| CMV IE1 SFC | 0.7 ± 0.8 | 1.6 ± 1.9 |
| EBV LMP2A SFC | 0.1 ± 0.2 | 0.2 ± 0.3 |
| EBV BMLF1 SFC | 0.03 ± 0.08 | 0.03 ± 0.07 |
CD4+ and CD8+ T-cell subsets were identified as naïve (NA; CD45RA+/CD62L+), central memory (CM; CD45RA−/CD62L+), effector-memory (EM; CD45RA−/CD62L−) and RA+ effector-memory (EMRA; CD45RA+/CD62L−) cells. The number of spot forming cells (SFC) enumerated in the ELISPOT assay at Day 0 following CMV or EBV peptide stimulation was adjusted for the total blood CD3+ cell count. Statistically significant difference from PRE indicated by (#p < 0.05). Data are mean ± SD.