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. 2016 May 10;4:e1992. doi: 10.7717/peerj.1992

Figure 2. Cordycepin regulates the LPS-induced matrix-degrading enzymes and extracellular matrix related gene expression in NP cells.

Figure 2

We used PCR (A–F) and an ELISA assay (G, H) to investigate the effect of cordycepin on the LPS-induced gene expression and matrix-degrading enzyme secretion of NP cells. (A–F) Real-time PCR indicated that cordycepin downregulated the LPS-induced gene over-expression of MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5. Moreover, cordycepin also counteracted the LPS-induced gene downregulation of collagen-2 and aggrecan especially at concentration of 50 mM or 100 µM. (G, H) An ELISA assay of MMP-3 and MMP-13 demonstrated that cordycepin inhibited LPS-induced MMPs secretion. The values are presented as the mean ± standard deviation. *P < 0.05 compared to the LPS group; **P < 0.01 compared to the LPS group; ##P < 0.01 compared to the control group.