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. 2016 May 10;4:e2004. doi: 10.7717/peerj.2004

Figure 3. Example of downstream analyses of DNA samples.

Figure 3

(A) Chromatogram of hydrolysed Daphnia DNA sample extracted with CTAB. (B) Chromatogram of hydrolysed Daphnia DNA sample extracted with MasterPure DNA purification kit. Shadowed area indicates 5-methylcytosine position. (C) Artificially methylated DNA generated from DNA extracted from Daphnia with MasterPure DNA purification kit. Sample was methylated using SssI methyltransferase (NEB, USA), treated with sodium bisulfite to preserve DNA methylation patterns, amplified using PCR and sequenced on the ABI 3730. (D) Unmethylated DNA generated from Daphnia with whole genome amplification (Sigma Aldrich, Dorset, UK) using MasterPure DNA purification kit. Shadowed area indicates cytosine-guanine position. (E) DNA fragment size selection (3 kb–9 kb) for mate pair library construction. (F) Quality scores for mate pair sequencing data.