Table 1. DNA yield measured by fluorescence-based method of quantification (SYBR Green I) represented by the mean ± SEM for ng of DNA per µg of Daphnia dry mass.

Missing values are due to salt precipitation from RNA later solution. Comparisons were made between the groups with same homogenisation method and with same extraction method. Same letters indicate the groups that differ statistically.

Preservation	Extraction method	Homogenisation method
		Ceramic bead homogenisation	Plastic pellet pestles	Proteinase K digestion
Liquid Nitrogen	Agencourt DNAdvance	5.40 ± 0.12a,b,d	4.10 ± 0.23c	2.77 ± 0.18d
	CTAB method	3.13 ± 0.71b	3.52 ± 0.79c	2.29 ± 0.17
	MasterPure DNA	5.45 ± 0.91a,b,e	3.10 ± 0.63e	2.93 ± 0.42e
	ZR genomic DNA	1.65 ± 0.10a	1.26 ± 0.09c	0.97 ± 0.05
RNAlater solution	Agencourt DNAdvance	4.07 ± 0.20	4.37 ± 0.08f	3.93 ± 0.20g
	CTAB method	2.26 ± 0.03	4.13 ± 0.87f	–
	MasterPure DNA	–	–	–
	ZR genomic DNA	2.07 ± 0.16	1.52 ± 0.16f	0.97 ± 0.03g

Notes.

P-values are indicated accordantly.

^ap < 0.0001.

^bp < 0.05.

^cp < 0.05.

^dp < 0.01.

^ep < 0.05.

^fp < 0.01.

^gp < 0.01.