Table III.
PCR primers used for the validation of the gene sequence variants.
| Gene symbol | Nucleotide changes | Primers (5′→3′) | Fragment size (bp) |
|---|---|---|---|
| MYH7 | c.2155C>T | Sense: GCTAATCAGTGACAAAGCCAGGATC Antisense: AGGGTGGAAGAGCCAACAGTAGC |
1,434 |
| PRKAG2 | c.298G>A | Sense: CAGTCCTGTGTGGTCAGAACTTGG Antisense: GGACCAGAAGGATTACGCTTTGAT |
907 |
| MYH7 | c.77C>T | Sense: AGCCAGCTTCTGCTCACTCCAG Antisense: GCCACTTGTAAGGGTTGACGGT |
1,013 |
| MYBPC3 | c.706A>G | Sense: CACCATACTTGGCTAATTTTCGT Antisense: GGATGACTGTTGACGGGACATAATGT |
1,542 |
| MYH7 | c.2654A>C | Sense: GCTAATCAGTGACAAAGCCAGGATC Antisense: AGGGTGGAAGAGCCAACAGTAGC |
1,434 |
| MYH6 | c.5410C>A | Sense: TGATGGAGGAGGGAAAGGTGATT Antisense: GGGTGCCAGGTGAACGGTTAA |
2,286 |
| MYH7 | c.1987C>T | Sense: GCAGAATCCATGTCCACCTGT Antisense: TGTCCTAGGAGGTCCTGTTCC |
1,248 |
| TNNT2 | c.887G>A | Sense: AGGGTGATTGTGAGGGTTACAG Antisense: GAGGGTCAAGAGAATGTGTCGT |
2,007 |