Table 2. Optimal Fold-activation Determined by Flow Cytometrya.
| cytoplasm | nucleus | peroxisomes | ER | |
|---|---|---|---|---|
| mCer3 | 44 (0) | 29 (0) | 55 (0) | 60 (0) |
| dL5** | 560 (500) | 720 (100) | 120 (100) | 920 (500) |
| dH6.2 | 760 (100) | 550 (100) | 300 (100) | 430 (500) |
| P13-CW | N.D. | 60 (500) | 79 (100) | 150 (500) |
a
Data show the fold activation relative to untransfected HEK293 cells labelled with the same MGe concentration (100, 200, or 500 nM, as indicated in parentheses). The optimal degree of activation was measured by comparing median fluorescent intensities of cell populations, in independent duplicate experiments, analyzing >20 000 cells per experiment.