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. 2016 Mar 19;67(10):3165–3175. doi: 10.1093/jxb/erw118

Fig. 5.

Fig. 5.

Generation of a Δgox knockout mutant in C. merolae. (A) Schematic presentation of the strategy to generate a knockout mutant for CmGOX. For detailed information see ‘Materials and methods’. (B) Verification of Δgox mutant lines #43, #45, and #46. PCR was performed on genomic DNA of the M4 background mutant and the Δgox mutant lines #43, #45, and #46 with primers flanking the CmGOX upstream and downstream regions (F1/R1) and the CmGOX coding region (F2/R2), respectively. Expected fragment sizes were F1/R1: 4.5kb (M4), 6kb (Δgox); F2/R2: 0.66kb (M4), – (Δgox). (C) Verification of absence of CmGOX transcripts in the Δgox knockout lines #43 and #46. RT-PCR analysis was performed on cDNA isolated from WT, M4 background mutant, and the Δgox mutant lines #43 and #46 with primers flanking the CmGOX coding region (F2/R2). Transcripts from the CMQ432C locus adjacent to the CmGOX locus were used as a control. Expected fragment sizes are CmGOX: 698bp; CMQ432C: 520bp.