Fig. 7.

Glycolate levels of WT and Δgox mutants #43 and #46 during the CO₂ shift experiment. Glycolate levels of the different strains were determined by GC-MS before the shift from HC (5% CO₂) to LC (0.04% CO₂) conditions, 3h and 24h after the shift to LC, and after a 24h recovery phase under HC conditions. Shown are means of four biological replicates and standard errors. n.d., not detectable. Significant differences were analysed by the non-parametric Mann–Whitney test. Significant differences to initial glycolate levels (HC) within one cell line are indicated by a (P < 0.05). Significant differences between the mutant and corresponding WT values are indicated by an asterisk (P < 0.05).