Fig. 5.

IGF1R-KD enhances CD24⁺ cancer cells plasticity in vivo. a Experimental strategy scheme is presented. CD24⁺ control or CD24⁺ IGF1R-KD cells were injected into the mammary fat pads, after 4 weeks, tumors were harvested and CD24 expression was evaluated. b CD24 expression in GFP-expressing cancer cells from mammary tumors was determined by FACS analysis. c Comparison of CD24⁺ population between control and IGF1R-KD tumors in WT and MKR mice. Mann-Whitney test was performed to compare between control and IGF1R-KD tumors, ^*** P < 0.001. FACS fluorescence-activated cell sorting, GFP green fluorescent protein, IGF1R insulin-like growth factor receptor, KD knockdown