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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Arch Biochem Biophys. 2016 Mar 29;597:30–47. doi: 10.1016/j.abb.2016.03.030

Figure 5. Growth hormone and HNF4α modulate gene expression accounts for most Group A Cyp1b1 responses.

Figure 5

(A) Growth hormone (GH) signaling integrated regulation of Nr0b2/Shp, HNF4α and PPARα-responsive genes showed a Cyp1b1-ko effect. (B) HNF4α-ko liver gene expression derived from ref 15 inversely correlates with Cyp1b1-ko responses from Group A genes (average from three mice per gene, represented as log base 2 FC). (C) As GH decreased with maturity, WT (solid line) gene expression decreased (Cyp7b1 and Cyp4a12a) or increased (Cyp2a4, 3a41, 4a10) between diet week (dw) 5 and dw11. Cyp1b1-ko (KO) prevents this change to produce observed Cyp1b1-ko differential expression relative to WT (ΔKO). (D) CAR, PXR, and PPAR-responsive Cyps display increased expression from dw5 to dw11 that was prevented in Cyp1b1-KO. Cyp7b1 and Cyp4a12a exhibit a decrease from dw5 to dw11, which is prevented by Cyp1b1-ko. Female expression is higher for the first three, male expression for the remaining pair. Cyp4f14 is unaffected by Cyp1b1 or diet at either dw5 or dw11. (E) Reported expression changes produced in Shp-ko mice [ref 42] correlate with multiple Cyp1b1-ko changes in both Group A and Group C.