Skip to main content
. 2016 May 10;57(6):2584–2592. doi: 10.1167/iovs.15-19006

Figure 5.

Figure 5

Exendin-4 attenuates the inflammatory response of BV2 microglial cells to LPS. We treated BV2 cells with Ex-4 (100 nmol/L) 1 hour prior to the LPS stimulus (100 ng/mL for 4 hours or 24 hours). Total RNA was isolated from the cells and the relative levels of mRNA of (A) IL-1β, (B) IL-6, (C) TNF, (D) CCL2, and (E) NOS2 were determined by duplex qRT-PCR with β-actin serving as control. Data are presented as the relative mRNA quantity and represent the mean ± SEM (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001 versus control. #P < 0.05, ##P < 0.01, ###P < 0.001 versus LPS. Two-way ANOVA followed by Bonferroni's post hoc test.