Table 1.

Analysis of E9.5 wild type and Fbln1 null proximal outflow tract cushions.

Metric	Wild typee	Fibulin-1 nullf	% Changeg	P valueh
Endothelial cell numbers (mean) proximal OFTa	26 ± 4.1	32 ± 5.1	23.1	0.11
Mesenchymal cell numbers (mean) proximal OFTa	3.5 ± 2.4	15.9 ± 2.24	354.3	0.0002
Total number of blood cells proximal OFTa	0	9.18 ± 10.74	NA	0.13
Total number of cells (endothelial, mesenchymal, blood cells)a	29.5 ± 6.2	57 ± 8.7	93.2	0.002
Ratio of mesenchymal:endothelial cellsa	0.12 ± 0.07	0.49 ± 0.07	308.3	0.0004
Proximal OFT cushion area (mm2)b	23 ± 5.6	18.4 ± 1.14	−21.4	0.13
Endothelial cells/mm2c	1.15 ± 0.35	1.73 ± 0.23	50.4	0.03
Mesenchymal cells/mm2c	0.16 ± 0.14	0.86 ± 0.15	437	0.028
Blood cells/mm2c	0	0.48 ± 0.56	NA	0.13
Total Cells (endothelial, mesenchymal, blood cells)/mm2c	1.32 ± 0.49	3.09 ± 0.35	134.1	0.001
Percent positive PHH3 cells (endothelial, mesenchymal cells)d	10 ± 3	14± 5	40.0	0.29

Mean values ± standard deviation are indicated.NA, calculation not applicable.

^aProximal OFT endothelial, mesenchymal cells and blood cell counts were counted in H&E stained tissue sections of E9.5 wild type and Fbln1 null embryos.

^bArea (arbitrary units) of the proximal OFT cushion was calculated using Image J software.

^cMeasurements obtained from the mean number of cells in proximal OFT and mean proximal OFT area.

^a,b,c The data were obtained from analysis of 54 sections from 4 wild type and 54 sections from 4 Fbln1 null embryos.

^dCalculated by counting the number of anti-PHH3 positive cells relative to the total number of nuclei using the Adobe Photoshop Count tool. PHH3 analysis was performed on 15 sections from 2 wild type and 15 sections from 2 Fbln1 null embryos.

^en = 4.

^fn = 4.

^gPercent change calculated as change in Fbln1 null relative to wild type.

^hP value was calculated using Student's t-test.

^a,d P value was calculated using Fisher's exact test.