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Western blot analysis of S9 protein. COS-1 cells were transfected with plasmids containing each of the mEH variant amino acids and 5 μg of S9 protein was electrophoretically separated, transferred to Immobilon-P, and reacted with antibody specific for mEH protein. The mEH standard is 100 ng of purified mEH protein. The size of the protein was estimated from molecular standards, run concurrently.
