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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: Clin Cancer Res. 2012 Nov 13;19(3):643–656. doi: 10.1158/1078-0432.CCR-12-1063

Figure 2. CXCR1/2 signalling regulates patient-derived mammosphere formation/self-renewal activity.

Figure 2

A Correlation between metastatic fluid IL-8 level and MFE, n=10 cancers (Pearson correlation coefficient, r = 0.652; P <0.05). Metastatic fluid IL-8 level was determined following removal of cellular matter using an ELISA. Details of MFE and metastatic fluid IL-8 level for each cancer are summarised in Table 1. B MFE of HER2 positive (n=6) and HER2 negative (n=11) patient-derived breast cancers after 7 days in non-adherent culture with/without IL-8 (100ng/ml). Controls were treated with vehicle. C Secondary mammosphere formation i.e. self-renewal of patient-derived breast cancers (HER2 positive n=2; HER2 negative n=4) treated with IL-8 (100ng/ml) in the first generation. Columns, fold change in mammosphere self-renewal normalised to control; bars, SEM. *, P < 0.05. D MFE of HER2 positive (n=4) and HER2 negative (n=11) cancers treated with either IL-8 (100ng/ml, grey bars), SCH563705 (100nM, white stripped bars), or SCH563705 and IL-8 (grey stripped bars) at the stated respective doses. Controls (white bars) were treated with vehicle. B and D Columns, fold change in MFE normalised to control; bars, SEM. ***, P < 0.001; NSD, no significant difference.