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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: Leukemia. 2015 May 14;29(10):1981–1992. doi: 10.1038/leu.2015.106

Figure 6.

Figure 6

Transient treatment of MV4-11 cells with MLN4924 is sufficient for the inception of monocytic differentiation. (a) Top: diagram of a timeline of the transient treatment with MLN4924. MV4-11 cells were treated with 100 nm MLN4924 for 24 h, washed in MLN4924-free medium three times and grown for up to an additional 6 days in the absence of MLN4924. Bottom: Wright–Giemsa-stained cytospin preparations of cells collected on day 6 (1+5) or day 7 (1+6) of the culture. (b) Whole-cell extracts were tested by western blot for c-myc protein levels and the total protein levels were normalized to actin. Densitometric quantification of band intensities, using ImageJ software is shown below. (c) Cells harvested on day 7 were analyzed by fluorescence-activated cell sorting using anti-CD-115 and anti-CD11c antibodies.