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. Author manuscript; available in PMC: 2016 May 16.
Published in final edited form as: J Proteome Res. 2015 Aug 24;14(10):4246–4256. doi: 10.1021/acs.jproteome.5b00398

Figure 1. Experimental overview.

Figure 1

Samples for TMT analysis were prepared as illustrated above. PaSC were harvested following 12 hr serum starvation and subsequent 12 hr treatment with either nicotine or α-bungarotoxin. Cells were harvested and lysed so that proteins could be extracted and prepared for TMT-based mass spectrometry analysis. The additional step of TiO2 enrichment was performed for phosphopeptide analysis. Control samples were labeled with 126, 127N, and 127C; nicotine-treated samples were labeled with 128N, 128C, and 129N, while α-bungarotoxin-treated samples were labeled with 129C, 130N, and 130C.