. Author manuscript; available in PMC: 2016 May 16.

Published in final edited form as: J Proteome Res. 2015 Aug 24;14(10):4246–4256. doi: 10.1021/acs.jproteome.5b00398

Table 2. Phosphorylation level analysis after the 12 hr treatment.

Category
Phosphopeptide enrichment	>86%
Quantified phosphorylation sites across all channels	16,622
Percentage of pS : pT : pY	86% : 12% : 2%
Singly phosphorylated peptides	11,999
Multiply phosphorylated peptides	4,623
Significantly altered phosphorylation levels^a
nicotine vs. control
up	59
down	73
α-bungarotoxin vs. control
up	314
down	137

p-value <0.01, fold change > ±1.5