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. 2015 Sep 9;309(11):C747–C758. doi: 10.1152/ajpcell.00085.2015

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Fig. 4. — Effects of NH₃/NH₄⁺ and MA/MA⁺ on intracellular pH (pH_i) and membrane potential (V_m) in oocytes expressing RhAG. Exposure of RhAG-expressing oocytes to NH₃/NH₄⁺ (5 mM) caused a rapid decrease in pH_i (dpH_i/dt = −11 × 10⁻⁴ pH units/s) and a large depolarization of the cell membrane (segment ab). Removal of bath NH₃/NH₄⁺ resulted in full recovery of pH_i and V_m (segment bc). On the other hand, exposure of the oocyte to MA/MA⁺ (5 mM) caused a significant increase in pH_i (dpH_i/dt = +7 × 10⁻⁴ pH units/s) but also significantly depolarized the cell (segment cd). Removal of MA/MA⁺ reversed these changes (segment de). S, slope. Tracings are representative results from 20 experiments.