Fig 2. Expression of the 4^th gene (escA) of lee3 is affected by the translation of mpc.

Abolishing the initiation codon of mpc reduced the expression level of EscA. (B) Translation of escN affects the immediate downstream translation of escA. The illustrations show the plasmids together with the Western blotting analyses and these are displayed in a manner similar to that described in legend to Fig 1. OmpC from the various individual samples was detected in parallel to ensure comparable sample loadings.