Fig 5. A region spanning the first 100 nucleotides of mpc is involved in the expression regulation of the downstream genes within lee3.

(A) An illustration of the constructs carrying an expression cassette similar to that of pV-N except that differently sized regions from the 3’-end of mpc are separately placed upstream to escV. (B) Western blotting analysis using anti-His_x6 is able to detect the amount of tagged EscN expressed by the individual constructs. Note: a dot-framed mpc fragment represents the 3’-end remaining mpc sequence that has been introduced without an in-frame ATG. The number above each frame represents the size (in base pairs) of the 3’-end fragment of mpc. The 5’-end nucleotides of mpc truncated are superscripted with “Δ.” A solid-line frame indicates that an ATG has been in-frame engineered in front of the mpc fragment containing nucleotides 52–354. Note: mpc^Δ51–100 represents a deletion of nucleotides 51–100 of mpc in the construct, and this construct contains no in-frame ATG since the first 50 nucleotides of the frame was derived from ^ACmpc. See legend to Fig 1 for additional explanations of the diagram.