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. 2016 May 16;11(5):e0155261. doi: 10.1371/journal.pone.0155261

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© 2016 Mansour et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Pooled semen and CVL was separated by size (>500 kDa) and charge (B) by high performance liquid chromatography (HPLC). (C) Unfractionated (UF, diluted 1:10) and charge-separated, high molecular weight (MW) semen and CVL protein peaks were then run on a reduced SDS-PAGE gel and Coomassie stained. TNC was detected with an anti-TNC monoclonal antibody. The 250 kD band staining on anti-TNC western blot was confirmed to be TNC by LC/MS.