Table 1. Candidate reference genes selected for stability analysis.
| Transcript | Function | Oligo sequence | Product size | Tma(°C) | Eb (%) |
|---|---|---|---|---|---|
| Succinate dehydrogenase (SDHA) | Respiratory chain and citric cycle enzyme | F: AAACCGGGAACGACCTTATC | 108bp | 58.4 | 94 |
| R: TCCAATCCTTCCAACTGTCC | |||||
| LF: AAACCGGGAACGACCTTATC | 449bp | 58.4 | 84 | ||
| LR: TCTCACGTGCCATCACAATC | |||||
| NADH dehydrogenase (NADH) | Respiratory chain enzyme | F: TCCGCTTAAGGAGCTTATCG | 72bp | 58.4 | 103 |
| R: ATCACGCACAGCAGGTTATC | |||||
| Large Ribosomal sub-unit (LRSU) | Structural protein in ribosome | F: ACGTATTCCATTCGGCTTCC | 106bp | 58.4 | 102 |
| R: GAGTCCGGCGAGGTATGAGT | |||||
| TATA-binding protein (TBP) | DNA transcription factor | F: AAGATCGTCAACGTGCTTGG | 74bp | 58.4 | 94 |
| R: TTGTTGGCCTGTGAGAAAGG | |||||
| LF: TCAACCCGTCTTCTTCCTTG | 551bp | 61.2 | 101 | ||
| LR:CAACCTCTGAGGCTCACAAAC | |||||
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | Glycolysis | F:CGCAAGGCCGGTGCCAAAAA | 62bp | 62.1 | 99 |
| R:ACGAACATTGGCGCATCGGGT | |||||
| 18S rRNA (18S) | Structural protein in ribosome | F: AATGCCATCATTACCATCCT | 60bp | 54.3 | 97 |
| R: CAAAAACCAATCGGCAATCT | |||||
| Heat shock protein 90 (HSP90) | Chaperone protein | F: TTTGTAACCGACACGAGCTG | 117bp | 58.4 | 92 |
| R: TGTTGAGCGTGTGAAGAAGC | |||||
| LF: GATTTCGAGGTGCTTCTTCG | 578bp | 58.4 | 81 | ||
| LR: ACATGAAGCTGGGAATCCAC | |||||
| Cyclophilin | Ubiquitous multi function protein | F: CCGGTAAAGCAGTCCACGA | 98bp | 59.5 | 103 |
| R: AGGCATCGACTTTCCTTTGG | |||||
| α-tubulin | Cytoskeletal structure | F: AATTAGTTGCTCGCCACGAT | 241bp | 56.4 | 104 |
| R: TGGCAAGAGGACTTCCCATA | |||||
| Actin | Cytoskeletal structure | F: TTGCTGACCGTATGCAGAAA | 100bp | 54.6 | 94 |
| R:CCGATCCAGACGGAATACTT |
a Tm is annealing melting temperature for oligos.
b E is efficiency of qPCR reaction as determined by standard curve slope.