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. 2016 May 16;10(5):e0004716. doi: 10.1371/journal.pntd.0004716

Fig 2. CatB-/- and WT BMDCs respond similarly to TLR-agonists or L. major.

Fig 2

BMDCs from WT and CatB-/- mice were stimulated with LPS (100ng/ml) and CpG (250ng/ml) or with live or killed L. major promastigotes (1:5 MOI) for 24h to determine secreted cytokines by ELISA (A and B) or for 6h to assess mRNA by RT-PCR (C and D). (A) Concentration of IL-6 and TNF in supernatants of BMDCs from WT (filled bars) and CatB-/- (empty bars) mice stimulated with LPS or CpG or (B) with live or killed L. major. (C) Level of expression of IL-12p40, IL-12p35, IL-6, IFNβ, IL-10 and TNF transcripts from BMDCs of WT (filled bars) and CatB-/- (empty bars) mice stimulate with LPS or CpG or (D) with live or killed L. major. The mRNA expression levels were normalized to the expression of the HPRT gene and calculated as the n-fold difference with the level of expression in unstimulated cells. Data depicted represent the mean and SEM of three independent experiments. No statistical differences were found.